Label-free trace detection of bio-molecules by liquid-interface assisted surface-enhanced Raman scattering using a microfluidic chip

Surface-enhanced Raman scattering (SERS), owing to its high sensitivity based on localized surface plasmon resonance of nanostructured metals, is recently attracting much attention be used for biotechnology, such as cell imaging and tumor therapy. On the other hand, trace detection bio-molecules with large molecular weight still challenging because troublesome treatment SERS substrate using coupling or cross-linking agents required. In this paper, we apply liquid interface assisted (LI-SERS) method, which provides unique features collection self-immobilization analyte molecules substrate, realize label-free limits pM ~ fM. Specifically, deoxyribonucleic acid (DNA) discrimination quantitative ?-Amyloid (A?) in trace-concentration are demonstrated illustrate ultrahigh versatility LI-SERS method. The results suggest promising early-stage diagnosis diseases virus infection Alzheimer's disease.