Labile carbon inputs support the recovery of bacterial communities, but not fungal communities, from a simulated bovine urine event

Abstract Inputs of carbon to soil may be used stimulate microbial growth and immobilize excess nitrogen from sources such as livestock urine. However, the responses taxa inputs under conditions remain poorly understood. Using DNA metabarcoding a field-based lysimeter experiment, we characterised temporal (up 112 days) bacterial fungal communities simulated bovine urine event plus labile (sucrose) at two concentrations. Fungal were impacted more strongly than by following event, with variable among taxa. Chytridiomycota Glomeromycota richness most negatively affected, Tremellomycetes positively inputs. A minority ASVs had greatly increased proportional abundances in response carbon, while trophic composition became highly dominated saprotrophs experiment end. Bacterial showed consistent trends declining (to about 14 recovering inputs, but carbon-related evenness abundance varied between Proportional Actinobacteria, Bacteroidetes, Betaproteobacteria, Gammaproteobacteria whereas Acidobacteria, candidate division WPS-1, Planctomycetes, Deltaproteobacteria, Verrucomicrobia decreased. These results show that limit nitrate leaching support recovery events have long-term impacts on community function, potential consequences for food webs, sequestration, agricultural productivity.