Lactose fermentation by engineered Saccharomyces cerevisiae capable of fermenting cellobiose
نویسندگان
چکیده
منابع مشابه
Engineered Saccharomyces cerevisiae capable of simultaneous cellobiose and xylose fermentation.
The use of plant biomass for biofuel production will require efficient utilization of the sugars in lignocellulose, primarily glucose and xylose. However, strains of Saccharomyces cerevisiae presently used in bioethanol production ferment glucose but not xylose. Yeasts engineered to ferment xylose do so slowly, and cannot utilize xylose until glucose is completely consumed. To overcome these bo...
متن کاملAcquisition of lactose-fermenting properties by salmonellae. I. Interrelationship between the fermentation of cellobiose and lactose.
The fermentation of lactose by salmonellae has seldom been accomplished, and in but few serological types (Kauffmann, 1954; Kristensen, 1948, 1955). In previous investigations we (Schafler and SchiLfler, 1959) succeeded in obtaining lactose-positive variants in 17 out of 30 salmonella strains belonging to different types, by means of 32 to 150 days of incubation on partially defined media conta...
متن کاملLeveraging transcription factors to speed cellobiose fermentation by Saccharomyces cerevisiae
BACKGROUND Saccharomyces cerevisiae, a key organism used for the manufacture of renewable fuels and chemicals, has been engineered to utilize non-native sugars derived from plant cell walls, such as cellobiose and xylose. However, the rates and efficiencies of these non-native sugar fermentations pale in comparison with those of glucose. Systems biology methods, used to understand biological ne...
متن کاملConstruction of a ̄occulent Saccharomyces cerevisiae fermenting lactose
A ̄occulent Saccharomyces cerevisiae strain with the ability to express both the LAC4 (coding for b-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces marxianus was constructed. This recombinant strain is not only able to grow on lactose, but it can also ferment this substrate. To our knowledge this is the ®rst time that a recombinant S. cervisiae has been found to fe...
متن کاملEvaluation of Ethanol Production Activity by Engineered Saccharomyces cerevisiae Fermenting Cellobiose through the Phosphorolytic Pathway in Simultaneous Saccharification and Fermentation of Cellulose.
In simultaneous saccharification and fermentation (SSF) for production of cellulosic biofuels, engineered Saccharomyces cerevisiae capable of fermenting cellobiose has provided several benefits, such as lower enzyme costs and faster fermentation rate compared with wild-type S. cerevisiae fermenting glucose. In this study, the effects of an alternative intracellular cellobiose utilization pathwa...
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ژورنال
عنوان ژورنال: Journal of Biotechnology
سال: 2016
ISSN: 0168-1656
DOI: 10.1016/j.jbiotec.2016.07.018