Loss of multipotency in adipose-derived stem cells after culture in temperature-responsive injectable polymer hydrogels

Abstract Adipose-derived stem cells (AdSCs), a type of mesenchymal cell, are expected to be applicable regenerative medicine and cellular delivery systems. The maintenance cell multipotency control the differentiation direction important for these applications. However, is widely believed depend on physical properties their scaffold. In this study, we explored whether AdSCs, that is, ability differentiate into multiple cells, maintained when they removed from injectable polymer (IP) hydrogels with various degrees cross-linking induced osteoblasts adipocytes. We confirmed AdSCs cultured in IP an undifferentiated state. adipocytes cannot ensured; specifically, may decrease hydrogels. When hydrogel extreme softness poor adhesion properties, remained state, but was reduced. These results provide insights systems using