Macromolecular structures without crystals
نویسندگان
چکیده
منابع مشابه
Macromolecular structures without crystals.
E lectron microscopy has many attractive capabilities as a tool for experimentally visualizing the structures of biological cells and macromolecules. Particles can be imaged while freely suspended in biochemically ‘‘native’’ buffers, and electrons can be focused to resolutions exceeding anything that a biochemist’s heart might desire. To be sure, biological electron microscopy also is subject t...
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The mother liquor from which a biomolecular crystal is grown will contain water, buffer molecules, native ligands and cofactors, crystallization precipitants and additives, various metal ions, and often small-molecule ligands or inhibitors. On average, about half the volume of a biomolecular crystal consists of this mother liquor, whose components form the disordered bulk solvent. Its scatterin...
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This paper presents a discussion of existing methods for the analysis of macromolecular interactions and complexes in crystal packing. Typical situations and conditions where wrong answers may be obtained in the course of ordinary procedures are presented and discussed. The more general question of what the relationship is between natural (in-solvent) and crystallized assemblies is discussed an...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2008
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.0800032105