Magnetic Nanoparticles to Unique DNA Tracers: Effect of Functionalization on Physico-chemical Properties

Abstract To monitor and manage hydrological systems such as brooks, streams, rivers, the use of tracers is a well-established process. Limited number potential salts, isotopes dyes, make study processes challenge. Traditional find limited due to lack multiplexed, multipoint tracing background noise, among others. In this regard, DNA based possess remarkable advantages including, environmentally friendly, stability, high sensitivity in addition showing great synthesis ideally unlimited unique capable tracing. prevent unintentional losses environment during application easy recovery for analysis, we hereby report encapsulation silica containing magnetic cores (iron oxide) two different shapes—spheres cubes. The iron oxide nanoparticles having size range 10–20 nm, have been synthesized using co-precipitation salts or thermal decomposition oleate precursor presence oleic acid sodium oleate. Physico-chemical properties size, zeta potential, magnetism etc. optimized ligands effective binding dsDNA, followed by silanization. We first time effect surface coating on at each stage functionalization, culminating shells. Efficiency three dsDNA molecules has studied quantitative polymerase chain reaction (qPCR). Our results show that our are excellent candidates monitoring with recoverability signal amplification. Graphic