Mammalian Two-Hybrid System: A Complementary Approach to the Yeast Two-Hybrid System
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چکیده
منابع مشابه
Mammalian two-hybrid system: a complementary approach to the yeast two-hybrid system.
Here we demonstrate the use of a mammalian two-hybrid system to study protein-protein interactions. Like the yeast two-hybrid system, this is a genetic, in vivo assay based on the reconstitution of the function of a transcriptional activator. In this system, one protein of interest is expressed as a fusion to the Gal4 DNA-binding domain and another protein is expressed as a fusion to the activa...
متن کاملYeast two-hybrid system
Introduction The two-hybrid system (THS), first introduced by Fields and Song (1989) (Ref. 1), is a powerful technique for identifying new proteins involved in specific biological processes. It allows for the rapid isolation of the gene that codes for a protein that interacts with a specific protein of interest. Here, we present not a review of the current THS technology but, rather, a comprehe...
متن کاملApplications of the yeast two-hybrid system.
In recent years, the yeast two-hybrid system has become the method of choice for detection and analysis of protein-protein interactions in an in vivo context. This system, which capitalizes on the significant genetic history and ease of protocols for manipulation of Saccharomyces cerevisiae, is accessible to most laboratories and is applicable to the pursuit of a large variety of experimental g...
متن کاملThe Two-Hybrid System
The bait and prey fusions are co-expressed in yeast where a physical interaction between X and Y leads to the reconstitution of a functional transcription factor that binds to the promoter of a reporter gene (Fig.1). Consequently, transcription of the reporter gene is switched on. A protein-protein interaction is therefore translated into reporter gene activity which can be detected or measured...
متن کاملThe yeast two-hybrid system: forward and reverse.
Dissecting the molecular mechanism of a biological process requires identifying the proteins and protein-protein interactions that mediate the process. To that end, investigators have seized upon the utility of the yeast two-hybrid system to directly assay interactions between known proteins (1) and to isolate novel interacting partners for a protein of interest (2). Once a protein-protein inte...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1997
ISSN: 0736-6205,1940-9818
DOI: 10.2144/97222pf02