Mass spectrometric analysis of asparagine deamidation and aspartate isomerization in polypeptides
نویسندگان
چکیده
منابع مشابه
Cxvi. the Enzymic Deamidation of Asparagine
THE occurrence of asparagine in the higher plant and its role in protein metabolism have formed the subject of numerous investigations. It has been found that the amount normally present in the green plant is very small and it is only under artificial conditions, associated with a carbohydrate deficiency, that an accumulation of this substance is ever obtained. E. Schulze [1906], in a series of...
متن کاملthe stady and analysis of rice agroclimatology in lenjan
the west of esfahan province, iran, is one of the most important agricultural areas throughout the country due to the climate variability and life-giving water of zayanderood river. rice is one of the major and economic crops in this area. the most important climatic elements in agricultural activities which should be considered include temperature, relative humidity, precipitation and wind. so...
15 صفحه اولSimultaneous Assessment of Asp Isomerization and Asn Deamidation in Recombinant Antibodies by LC-MS following Incubation at Elevated Temperatures
The degradation of proteins by asparagine deamidation and aspartate isomerization is one of several chemical degradation pathways for recombinant antibodies. In this study, we have identified two solvent accessible degradation sites (light chain aspartate-56 and heavy chain aspartate-99/101) in the complementary-determining regions of a recombinant IgG1 antibody susceptible to isomerization und...
متن کاملthe analysis of the role of the speech acts theory in translating and dubbing hollywood films
از محوری ترین اثراتی که یک فیلم سینمایی ایجاد می کند دیالوگ هایی است که هنرپیش گان فیلم میگویند. به زعم یک فیلم ساز, یک شیوه متأثر نمودن مخاطب از اثر منظوره نیروی گفتارهای گوینده, مثل نیروی عاطفی, ترس آور, غم انگیز, هیجان انگیز و غیره, است. این مطالعه به بررسی این مسأله مبادرت کرده است که آیا نیروی فراگفتاری هنرپیش گان به مثابه ی اعمال گفتاری در پنج فیلم هالیوودی در نسخه های دوبله شده باز تولید...
15 صفحه اولTransamination and associated deamidation of asparagine and glutamine.
With this system, no deamidation of glutamine occurred in the absence of a-keto acid, and, with all keto acids tested except two (pyruvate and a-ketobutyrate), transamination was absent or extremely slow when glutamate was substituted for glutamine. Experiments with W-labeled glutamine indicated that the ammonia evolved during this reaction was derived from t.he amide group of glutamine. Althou...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: ELECTROPHORESIS
سال: 2010
ISSN: 0173-0835
DOI: 10.1002/elps.201000027