Mass Spectrometric Method for Analyzing Metabolites in Yeast with Single Cell Sensitivity

Supporting Information Mass Spectrometric Method for Analyzing Metabolites in Yeast with Single Cell Sensitivity

Mass spectrometric method for analyzing metabolites in yeast with single cell sensitivity.

Single-Cell Level Mass Spectrometric Imaging

Secondary ion mass spectrometry (SIMS) is capable of imaging biological molecules across single cells with several hundred nanometer lateral resolution, and has the potential to examine the functional segregation of lipids in cell membranes. Lipids are believed to play a crucial role in fundamental biological functions, such as intercellular communication. For example, high curvature lipids may...

Mass spectrometric method incorporating enzymatic amplification for attomole-level analysis of target metabolites in biological samples.

We report a sensitive method for selective detection of target metabolites from the central metabolic pathway by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS), in which the MS signal is enhanced by up to three orders of magnitude in the course of in situ enzymatic amplification.

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چکیده زبان سیستم کاملی ازقوانین است که برای ایجاد ارتباط و تبادل معانی مورد استفاده قرار می گیرد که در رابطه تنگاتنگ با روابط قدرتی افراد می باشد. این رابطه و قدرت افراد می تواند مستقیما در انتخاب ضمایر و سایر ساختارهای زبانی حتی در رفتار افراد تاثیرگذار باشد. در هرجامعه ای ، تقریبا نوعی روابط قدرتی درمیان افراد موجود می باشد و این رابطه درنحوه ی استفاده ی آنها در زبان منعکس می شود. بررسی ای...