Mechanism of ribosome rescue by alternative ribosome-rescue factor B
نویسندگان
چکیده
منابع مشابه
Mechanism of ribosome rescue by ArfA and RF2
ArfA rescues ribosomes stalled on truncated mRNAs by recruiting release factor RF2, which normally binds stop codons to catalyze peptide release. We report two 3.2 Å resolution cryo-EM structures - determined from a single sample - of the 70S ribosome with ArfA•RF2 in the A site. In both states, the ArfA C-terminus occupies the mRNA tunnel downstream of the A site. One state contains a compact ...
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In eukaryotes, Dom34 is involved in the rescue of ribosomes that stall on mRNAs during protein synthesis. Using ribosome profiling, Guydosh and Green reveal that, in addition to rescue of ribosomes stalled on truncated mRNAs, Dom34 also recycles ribosomes that are unexpectedly found in the 3' untranslated regions of many cellular mRNAs.
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Ribosomes synthesizing proteins containing consecutive proline residues become stalled and require rescue via the action of uniquely modified translation elongation factors, EF-P in bacteria, or archaeal/eukaryotic a/eIF5A. To date, no structures exist of EF-P or eIF5A in complex with translating ribosomes stalled at polyproline stretches, and thus structural insight into how EF-P/eIF5A rescue ...
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The tmRNA system performs translational surveillance and ribosome rescue in all eubacteria and some eukaryotic organelles. This system intervenes when ribosomes read to the 3' end of an mRNA or pause at internal codons with subsequent mRNA cleavage. A complex of alanyl-tmRNA (which functions as a tRNA and mRNA), SmpB protein, and EF-TucGTP binds stalled ribosomes, the nascent polypeptide is tra...
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ژورنال
عنوان ژورنال: Nature Communications
سال: 2020
ISSN: 2041-1723
DOI: 10.1038/s41467-020-17853-7