Mechanisms of protein localization

A New Paradigm in Biology – The Effect of Localization of a Protein on its Anti-genicity

Ultrastructural Study of Rotavirus Replication and Localization of the Intermediate Capsid Protein VP6

Rotavirus, a triple-layered non-enveloped member of the Reoviridae family, obtained a transient membrane envelope when newly synthesized subviral particles bud into the endoplasmic reticulum (ER). As rotavirus particles mature, they lose their transient membrane and obtain outer layer. It is mostly believed that only double layered particles bud into the ER. The present study describes that the...

Mechanisms of Subcellular mRNA Localization

Localization of RNA is a widespread and efficient way to target gene products to a specific region of a cell or embryo. This strategy of posttranscriptional gene regulation utilizes a variety of distinct mechanisms to regulate the movement and anchoring of different transcripts.

Intracellular Localization of FLAG-Peroxisomal Protein in Chinese Hamster Ovary (CHO) Cells

Epitope tagging is a method of expressing proteins whereby an epitope for a specific monoclonal antibody is fused to a target protein using recombinant DNA techniques. The aim of this study was to sub-clone the peroxisomal protein (PEP) cDNA into a mammalian expression vector leading to the formation of a  chimeric PEP-cDNA containing the FLAG epitope. The FLAG-PEP recombinant cDNA was construc...

Fouling mechanisms during protein microfiltration: The effects of protein structure and filtration pressure on polypropylene microporous membrane performance

A polypropylene microporous membrane (PPMM) was fabricated by thermally induced phase separation (TIPS) method. The effects of protein size and structure as well as filtration pressure on the membrane performance and fouling mechanisms were investigated using two different proteins, bovine serum albumin (BSA) and collagen, in dead-end filtration setup. Obtained results showed that, for each pro...