Mechanisms of Sequence Dependent Translational Stalling
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چکیده
منابع مشابه
Mechanisms of Sequence Dependent Translational Stalling
bundles undergo deformations highly similar to a twisted rod under compression. Our data suggests that tau-like proteins minimize microtubule lattice interactions and the prevent torque generation that leads to extreme neuron deformations. These experimental results, together with mechanical modeling of the neuron, suggest that spectrin tension and microtubule bundle mechanics are crucial for s...
متن کاملThe dynamics of SecM-induced translational stalling.
SecM is an E. coli secretion monitor capable of stalling translation on the prokaryotic ribosome without cofactors. Biochemical and structural studies have demonstrated that the SecM nascent chain interacts with the 50S subunit exit tunnel to inhibit peptide bond formation. However, the timescales and pathways of stalling on an mRNA remain undefined. To provide a dynamic mechanism for stalling,...
متن کاملTranslational stalling at polyproline stretches is modulated by the sequence context upstream of the stall site
The polymerization of amino acids into proteins occurs on ribosomes, with the rate influenced by the amino acids being polymerized. The imino acid proline is a poor donor and acceptor for peptide-bond formation, such that translational stalling occurs when three or more consecutive prolines (PPP) are encountered by the ribosome. In bacteria, stalling at PPP motifs is rescued by the elongation f...
متن کاملMechanisms of SecM-mediated stalling in the ribosome.
Nascent-peptide modulation of translation is a common regulatory mechanism of gene expression. In this mechanism, while the nascent peptide is still in the exit tunnel of the ribosome, it induces translational pausing, thereby controlling the expression of downstream genes. One example is SecM, which inhibits peptide-bond formation in the ribosome's peptidyl transferase center (PTC) during its ...
متن کاملMechanisms of ribosome stalling by SecM at multiple elongation steps
Regulation of translating ribosomes is a major component of gene expression control network. In Escherichia coli, ribosome stalling by the C-terminal arrest sequence of SecM regulates the SecA-dependent secretion pathway. Previous studies reported many residues of SecM peptide and ribosome exit tunnel are critical for stalling. However, the underlying molecular mechanism is still not clear at t...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2017
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2016.11.199