Mechanistic modeling of ligand density variations on anion exchange chromatography
نویسندگان
چکیده
منابع مشابه
Anion-Exchange Chromatography of Phosphopeptides: Weak Anion Exchange versus Strong Anion Exchange and Anion-Exchange Chromatography versus Electrostatic Repulsion–Hydrophilic Interaction Chromatography
Most phosphoproteomics experiments rely on prefractionation of tryptic digests before online liquid chromatography-mass spectrometry. This study compares the potential and limitations of electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) and anion-exchange chromatography (AEX). At a pH higher than 5, phosphopeptides have two negative charges per residue and are well-retained...
متن کاملDevelopment of a new method based on chiral ligand-exchange chromatography for the enantioseparation of propranolol
A new chromatographic procedure was proposed for the separation of propranolol (PRN) enantiomers based upon enantioselective chiral ligand-exchange chromatography. The separation was carried out on a short C8 column leading to considerably short separation time. L-alanine and Cu2+ were applied as chiral selector and central bivalent complexing ion, respectively. It was found that the kind of co...
متن کاملLigand Exchange Chromatography
of amino alcohols using Z-L-Glu-L-Pro or Z-L-Glu-D-Pro as chiral counter ions and Hypercarb as the solid phase. Chirality 9: 650}655. Knox JH and Jurand J (1982) Separation of optical isomers by zwitterion pair chromatography. Journal of Chromatography 234: 222}224. Knox JH and Ross P (1997) Carbon-based packing materials for liquid chromatography. Advances in Chromatography, 74}162. Petterson ...
متن کاملDevelopment of a new method based on chiral ligand-exchange chromatography for the enantioseparation of propranolol
A new chromatographic procedure was proposed for the separation of propranolol (PRN) enantiomers based upon enantioselective chiral ligand-exchange chromatography. The separation was carried out on a short C8 column leading to considerably short separation time. L-alanine and Cu2+ were applied as chiral selector and central bivalent complexing ion, respectively. It was found that the kind of co...
متن کاملPurification of DNA by anion-exchange chromatography.
Column chromatography has evolved to provide a rapid and effective alternative to more laborious methods for preparing high-quality DNA, such as CsCl-gradient centrifugation. This unit describes the use of a column made of a unique anion-exchange resin that selectively binds nucleic acids, allowing rapid separation of DNA from contaminating RNA, proteins, carbohydrates, and metabolites. The pro...
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ژورنال
عنوان ژورنال: Journal of Separation Science
سال: 2020
ISSN: 1615-9306,1615-9314
DOI: 10.1002/jssc.202001077