Methionine supply during the peripartum period and early lactation alter immunometabolic gene expression in cytological smear and endometrial tissue of holstein cows

The objective of the present study was to evaluate effect feeding rumen-protected methionine (RPM) during peripartal period and early lactation on mRNA gene expression profiles uterine cytological smear endometrial samples Holstein cows (n = 20). Treatments consisted a supplementation with RPM [MET; n 11; at rate 0.08 % DM: Lys:Met 2.8:1, (Smartamine® M Adisseo, Alpharetta, GA, USA)] no (CON; 9; 3.5:1). Uterine cytology smears were collected 15, 30, 73 days in milk (DIM) analyzed for genes related metabolism, inflammation, metabolism. Regarding samples, tended increase adenosyltransferase 1 alpha (MAT1A) increased fibroblast growth factor 7 (FGF7), an time latter. On other hand, decreased glucose transporter 4 (GLUT4), interleukin beta (IL-1β), 6 (IL-6), 8 (IL-8), prostaglandin E synthase 3 (PTGES3), translocator protein 18 kDa (TSPO), mucin (MUC1) superoxide dismutase (SOD1) samples. There all variables except MAT1A, decreasing over time. TRT × TIME interaction GLUT4 expression, higher fed CON than 15 tendency 30 when compared RPM. For tissue lecithin-cholesterol acyltransferase (LCAT), S-adenosyl-l-homocysteine hydrolase (SAAH), FGF7, GLUT4, apolipoproteins (APOL3), APOL3 where its have IL1β expression. In conclusion, transition is beneficial immune response metabolism as indicated by favorable expressions affecting immunometabolism such challenging period.