Microbead-Beating Extraction of Polycyclic Aromatic Compounds from Seabird Plasma and Whole Blood

Seabirds are widely regarded as an invaluable bioindicator of environmental health. Matrices including eggs and feathers have been used non-lethal means to assess contaminant burdens. We developed a new approach for extraction polycyclic aromatic compounds (PACs) from seabird plasma serum based on automated microbead-beating homogenization extraction. Commercially available bovine were purposely fortified with suite PACs separately at three dosing levels, placed inside custom-made stainless-steel tube containing ceramic microbeads, subjected process using Precellys tissue homogenizer. Tubes shaken forcefully in three-dimensions, facilitating high mass-transfer the matrix into hexane solvent. The accuracy method ranged 55 120% limits detection quantitation 0.1 8 0.2 27 pg/μL, respectively. exhibited good repeatability both inter- intra-day < 30%. represents effective efficient important biological matrices.