Microbial community dynamics and their relationships with organic and metal pollutants of sugarcane molasses-based distillery wastewater sludge

Distillery sludge is a major source of aquatic pollution, but little known about their microbial community and association with the organic metal pollutants. Sugarcane molasses-based distillery an important industry in India, although waste usually treated prior to disposal, treatment often inadequate. The adverse effects pollutants sugarcane on biodiversity abundance disposal site have not been elucidated. This study aims address this gap knowledge. Samples were collected from discharge point, 1 2 km downstream (D1, D2, D3, respectively) Uttar Pradesh, physico-chemical properties characterised. Using QIIME, taxonomic assignment for V3 V4 hypervariable regions 16 S rRNA was performed. phyla Proteobacteria (28–39%), Firmicutes (20–28%), Bacteriodetes (9–10%), Actinobacteria (5–10%), Tenericutes (1–9%) Patescibacteria (2%) predominant bacteria all three sites. Euryechaeota, detected sites D1 D2 (1–2%) absent D3. Spirochaetes (5%), Sinergistetes Cloacimonetes (1%) only samples D1. Shannon, Simpson, Chao1, Observed-species indices indicated that (10.18, 0.0013, 36706.55 45653.84, has higher bacterial diversity richness than (6.66, 0.0001, 25987.71 49655.89, D3 (8.31, 0.002, 30345.53 30654.88, respectively), suggesting provided stressors favour survival can biodegrade detoxify them sludge. confirmed sufficiently effective new metagenomic information its impact surrounding community. It also offered insights into potential bioremediation candidates.