Microfluidic continuous flow digital loop-mediated isothermal amplification (LAMP)
نویسندگان
چکیده
منابع مشابه
Microfluidic continuous flow digital loop-mediated isothermal amplification (LAMP).
Digital nucleic acid detection is rapidly becoming a popular technique for ultra-sensitive and quantitative detection of nucleic acid molecules in a wide range of biomedical studies. Digital polymerase chain reaction (PCR) remains the most popular way of conducting digital nucleic acid detection. However, due to the need for thermocycling, digital PCR is difficult to implement in a streamlined ...
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We present eLAMP, a PERL script, with Tk graphical interface, that electronically simulates Loop-mediated AMPlification (LAMP) allowing users to efficiently test putative LAMP primers on a set of target sequences. eLAMP can match primers to templates using either exact (via builtin PERL regular expressions) or approximate matching (via the tre-agrep library). Performance was tested on 40 whole ...
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Herpetic keratitis which is resulted from herpes simplex virus type I, is considered the most common cause of corneal blindness that needs being diagnosed and treated in order to prevent irreversible eye effects. The best method to diagnose it is molecular method which has high sensitivity and exclusivity. The current study was done on 25 eye samples collected from patients referred to the Feyz...
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مقدمه و هدف: نیاز به روشی ساده تر، با کارایی بالاتر، سریعتر و ارزانتر نسبت به روشهای موجود جهت تشخیص بیماریهای دامپزشکی روش واکنش زنجیره ای پلی مراز (PCR) ، روشی سریع و حساس می باشد که قادر است تعداد نسخه اندکی از DNA هدف را تا سطحی که توسط ژل الکترفورز قابل تشخیص باشد، تکثیر نماید.بعلت کارایی بالا و قدرت زیاد PCR ، این روش در سالهای اخیر بطور گسترده ای در تشخیص پاتوژن های مختلف بیماریزا اعم ا...
متن کاملDetermination of Herpetic Keratitis Frequency Using Loop Mediated Isothermal Amplification (LAMP) Method in Isfahan, Iran
Herpetic keratitis which is resulted from herpes simplex virus type I, is considered the most common cause of corneal blindness that needs being diagnosed and treated in order to prevent irreversible eye effects. The best method to diagnose it is molecular method which has high sensitivity and exclusivity. The current study was done on 25 eye samples collected from patients referred to the Feyz...
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ژورنال
عنوان ژورنال: Lab on a Chip
سال: 2015
ISSN: 1473-0197,1473-0189
DOI: 10.1039/c4lc01158a