Microimaging Characterization of a B16-F10 Melanoma Metastasis Mouse Model

Prenyl Ammonium Salts – New Carriers for Gene Delivery: A B16-F10 Mouse Melanoma Model

PURPOSE Prenyl ammonium iodides (Amino-Prenols, APs), semi-synthetic polyprenol derivatives were studied as prospective novel gene transfer agents. METHODS AP-7, -8, -11 and -15 (aminoprenols composed of 7, 8, 11 or 15 isoprene units, respectively) were examined for their capacity to form complexes with pDNA, for cytotoxicity and ability to transfect genes to cells. RESULTS All the carriers...

Antitumor effect of RGD-4C-GG-D(KLAKLAK)2 peptide in mouse B16(F10) melanoma model.

Vasculature targeting agents have been tested as cancer therapeutics for the past few years. Such therapy could be accomplished using, for example, bifunctional (two-domain) peptides. RGD-4C-GG-D(KLAKLAK)2, a peptide designed by Ellerby and coworkers (1999) (full sequence: ACDCRGDCFCGGKLAKLAKKLAKLAK), binds selectively to alphaVbeta3 integrin receptors expressed in tumor neovasculature and, aft...

Functional roles of hyaluronan in B16-F10 melanoma growth and experimental metastasis in mice.

Although hyaluronan (HA), a high molecular weight glycosaminoglycan, is generally believed to regulate tumor growth, invasion, and metastasis, functional roles of HA have only been speculated indirectly from the outcome of blocking HA receptors (e.g., CD44). Using a phage display technique, we recently developed a synthetic peptide (GAHWQFNALTVR; Pep-1) that binds to and inhibits the function o...

Use of Mycobacterium vaccae (SRLI72) as an Immunomodulating Agent in Association with Murine Melanoma Model B16-F10

The Use of Anchored Agonists of Phagocytic Receptors for Cancer Immunotherapy: B16-F10 Murine Melanoma Model

The application of the phagocytic receptor agonists in cancer immunotherapy was studied. Agonists (laminarin, molecules with terminal mannose, N-Formyl-methioninyl-leucyl-phenylalanine) were firmly anchored to the tumor cell surface. When particular agonists of phagocytic receptors were used together with LPS (Toll-like receptor agonist), high synergy causing tumour shrinkage and a temporary or...