Micronucleus and FTIR spectroscopy analysis as screening tests for HPV vírus detection

Human Papillomavirus (HPV) infection is the main precursor of cervical cancer. Pap Smear diagnostic method this neoplasm by cytological analysis, however presents a high rate false-negative cases. This study aimed to verify if micronucleus (MN) frequency test and Fourier Transform Infrared (FTIR) Spectroscopy analysis may be alternative useful techniques detect HPV in fluid. Fifty samples with normal cytology obtained from patients after cytopathological examination were divided into two groups based on presence or absence previous molecular biology. Of fifty analyzed, 46% showed MN cells. The observed was higher positive related age, use oral contraceptives alcoholic beverages (p <0.001). FTIR spectra peak DNA proteins (1170cm-1; 1516cm-1; 1404cm-1; 1473cm-1) compared MN, probably due chromosomal histones constituents. However, no statistically significant difference between these considering that screening not specific. Thus, technique it possible classify spectral differences presented. can considered for detection HPV, being clinical practice because practical, simple, low-cost non-invasive method. important carry out studies larger sample investigate application spectroscopy diagnosis prevention