Micropatterned Structural Control Suppresses Mechanotaxis of Endothelial Cells

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Micropatterned structural control suppresses mechanotaxis of endothelial cells.

Vascular endothelial cell migration is critical in many physiological processes including wound healing and stent endothelialization. To determine how preexisting cell morphology influences cell migration under fluid shear stress, endothelial cells were preset in an elongated morphology on micropatterned substrates, and unidirectional shear stress was applied either parallel or perpendicular to...

متن کامل

Repositioning of cells by mechanotaxis on surfaces with micropatterned Young's modulus.

Adherent cells are strongly influenced by the mechanical aspects of biomaterials, but little is known about the cellular effects of spatial variations in these properties. This work describes a novel method to produce polymeric cell culture surfaces containing micrometer-scale regions of variable stiffness. Substrates made of acrylamide or poly(dimethylsiloxane) were patterned with 100- or 10-m...

متن کامل

The role of the dynamics of focal adhesion kinase in the mechanotaxis of endothelial cells.

The migration of vascular endothelial cells (ECs) is critical in vascular remodeling. We showed that fluid shear stress enhanced EC migration in flow direction and called this "mechanotaxis." To visualize the molecular dynamics of focal adhesion kinase (FAK) at focal adhesions (FAs), FAK tagged with green fluorescence protein (GFP) was expressed in ECs. Within 10 min of shear stress application...

متن کامل

Geometrically controlled endothelial tubulogenesis in micropatterned gels.

We present a novel approach to control endothelial tubulogenesis by spatially patterning cells within micromolded collagen gels. Endothelial cells cultured within microscale channels that were filled with collagen gel organized into tubes with lumens within 24-48 h of seeding. These tubes extended up to 1 cm in length, and exhibited cell-cell junction formation characteristic of early stage cap...

متن کامل

Modelling of migration and orientation of endothelial cells on micropatterned polymers

Understanding the cell migration on micropatterned polymers, as well as their orientation is a critical issue in tissue engineering, since it is the preliminary step in the blood vessel formation. In this paper, we derive an agent-based model to describe the migration and the orientation of endothelial cells seeded on bioactive micropatterned polymers. The model is obtained thanks to the experi...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Biophysical Journal

سال: 2008

ISSN: 0006-3495

DOI: 10.1529/biophysj.107.127761