"“Mitochondrial Cells” Can Live Without a Nucleus, and Possess Proteins Encoded by Both Nuclear and Mitochondrial DNA"

Nakano first discovered the “Mitochondrial cells” (Mito Cells), which can survive for long term despite lacking a nucleus. However, there was no clear evidence to indicate whether MitoCells were alive. To resolve this question, we constructed GFP-actin induced Mito Cell (GFP-actin Cell). Herein, report molecular analysis of both and MitoCells. Our results suggest that have minimum amounts proteins DNA needed sustain life.