Model of Membrane Fusion Driven by SNARE Clusters
نویسندگان
چکیده
منابع مشابه
A reduced SNARE model for membrane fusion.
Let's get together: A minimal model system was developed to mimic the SNARE-protein-mediated fusion of biological membranes (see picture). Fusion between two populations of liposomes is controlled by a pair of complementary lipidated oligopeptides that form noncovalent coiled-coil complexes and thereby force the membranes into close proximity to promote fusion. The model system displays the key...
متن کاملTesting the SNARE/SM protein model of membrane fusion.
I n eukaryotic cells, the budding and fusion of membranes mediates diverse but essential processes, ranging from cell division to organelle biogenesis to neurotransmitter secretion. All intracellular membrane fusion except for mitochondrial fusion is driven by SNARE and SM (Sec1/Munc18-like) proteins (1–3). Membrane fusion has been particularly intensely studied for neurotransmitter secretion. ...
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Neuronal SNARE proteins mediate neurotransmitter release at the synapse by facilitating the fusion of vesicles to the presynaptic plasma membrane. Cognate v-SNAREs and t-SNAREs from the vesicle and the plasma membrane, respectively, zip up and bring about the apposition of two membranes attached at the C-terminal ends. Here, we demonstrate that SNARE zippering can be modulated in the midways by...
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Using total internal reflection fluorescence microscopy, we have developed an assay to monitor individual fusion events between proteoliposomes containing vesicle soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) and a supported planar bilayer containing cognate target SNAREs. Approach, docking, and fusion of individual vesicles to the target membrane were quantifi...
متن کاملVariable cooperativity in SNARE-mediated membrane fusion.
The soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex drives the majority of intracellular and exocytic membrane fusion events. Whether and how SNAREs cooperate to mediate fusion has been a subject of intense study, with estimates ranging from a single SNARE complex to 15. Here we show that there is no universally conserved number of SNARE complexes involved ...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2011
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2010.12.1978