Model systems for single molecule polymer dynamics

Model systems for single molecule polymer dynamics.

Double stranded DNA (dsDNA) has long served as a model system for single molecule polymer dynamics. However, dsDNA is a semiflexible polymer, and the structural rigidity of the DNA double helix gives rise to local molecular properties and chain dynamics that differ from flexible chains, including synthetic organic polymers. Recently, we developed single stranded DNA (ssDNA) as a new model syste...

Single Stranded Dna: a New Model System for Single Molecule Polymer Dynamics by Christopher A. Brockman Thesis

Probing single molecule dynamics.

The room temperature dynamics of single sulforhodamine 101 molecules dispersed on a glass surface are investigated on two different time scales with near-field optics. On the 10(-2)- to 10(2)-second time scale, intensity fluctuations in the emission from single molecules are examined with polarization measurements, providing insight into their spectroscopic properties. On the nanosecond time sc...

Single-molecule enzymatic dynamics.

Enzymatic turnovers of single cholesterol oxidase molecules were observed in real time by monitoring the emission from the enzyme's fluorescent active site, flavin adenine dinucleotide (FAD). Statistical analyses of single-molecule trajectories revealed a significant and slow fluctuation in the rate of cholesterol oxidation by FAD. The static disorder and dynamic disorder of reaction rates, whi...

A Systems Dynamics Model for Project Management systems of Project-Based Organization

It is obvious that the success of a project-based organization is dependent on its projects. A variety of tools such as the project excellence model, project management maturity models, the earned value method, have been developed in this regard, but there are still delays in projects because the projects have dynamic nature with non-linear relationships and feedback processes during the projec...