Modified enzyme multiplied immunoassay technique of methotrexate assay to improve sensitivity and reduce cost
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چکیده
منابع مشابه
Adaptation of the enzyme-multiplied immunoassay for methotrexate to the centrifugal analyzer.
We adapted the homogeneous enzyme-multiplied immunoassay for methotrexate to the centrifugal analyzer. The sensitivity of the assay has been extended from 200 nmol/L down to 12.5 nmol/L. Several protocols may be followed, depending on whether the samples to be tested are from patients on low- or high-dose methotrexate regimens. Precision, accuracy, and sensitivity were satisfactory. Comparison ...
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Sixty-seven digoxin-containing sera were analyzed by both radioimmunoassay and EMIT. After some important modifications of the EMIT method, agreement between the two methods was very good. Reproducibility of the EMIT assay was excellent; daily variations in values found for control sera were quite small, and recovery of added digoxin was good. Slight hemolysis hadnegligible effects, but highly ...
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The clinical value and sensitivity of serum caffeine clearance measurement has been evaluated as an indicator of hepatic disease. After a 17 hour caffeine exclusion period, 300 mg of caffeine citrate was administered orally to the study subjects. Serum samples were taken four and 16 hours later. Serum caffeine concentrations were measured using an enzyme multiplied immunoassay technique (EMIT) ...
متن کاملEnzyme immunoassay and enzyme inhibition assay of methotrexate, with use of the centrifugal analyzer.
Methotrexate was determined by the homogeneous enzyme immunoassay (EMIT) with the Multistat and CentrifiChem centrifugal analyzers and by the enzyme inhibition assay with use of the Multistat centrifugal analyzer. With both methods, the standard curve extends from 0.2 to 2.0 mumol/L and there is no interference from bilirubin in concentrations up to 100 mg/L. Moderately lipemic samples do not i...
متن کاملComparison of enzyme-multiplied immunoassay technique with fluorescence polarization immunoassay for determination of gentamicin and tobramycin levels in serum.
We assayed serum gentamicin and tobramycin specimens by the enzyme multiplied immunoassay technique (Syva EMIT) and the fluorescence polarization immunoassay (Abbott TDx). When interassay and intraassay control samples were evaluated, both methods gave an overall coefficient of variation of less than +/- 10%. Using patient serum samples, we obtained excellent correlation with both methods in th...
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ژورنال
عنوان ژورنال: BMC Pharmacology and Toxicology
سال: 2019
ISSN: 2050-6511
DOI: 10.1186/s40360-018-0283-5