Modified Pagano Levin Medium to Isolate Candida Species

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Modified Pagano Levin medium to isolate Candida species.

The use of Pagano Levin medium for primary isolation of Candida species from highly contaminated specimens proved unsatisfactory owing to overgrowth by bacteria and fungi. Addition of cycloheximide and chloramphenicol to the medium greatly improved results by inhibiting overgrowth. The additional inhibitors also altered rate of growth and degree of pigmentation reported for some of the Candida ...

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Pagano-Levin Candida test medium: evaluation using vaginal samples.

Since 1958 pagano-Levin medium (PLM) has been used as an aid in the identification of Candida albicans. However, no statistical analysis of its effectiveness based on large number of random human specimens has been reported. The present study compared PLM (now called Candida test) with Sabouraud's plus antimicrobials and Littman's ox-gall agar without antimicrobials. Of 500 random vaginal sampl...

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Comparison of Sabouraud dextrose and Pagano-Levin agar media for detection and isolation of yeasts from oral samples.

The sensitivities of Sabouraud dextrose agar and modified Pagano-Levin agar for the primary isolation of yeasts and the recovery of multiple yeast species from single clinical samples were compared by using oral-rinse samples. Although there was a highly significant positive correlation between the numbers of yeasts recovered from both media, modified Pagano-Levin agar was far superior in detec...

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Performance of candida ID, a new chromogenic medium for presumptive identification of Candida species, in comparison to CHROMagar Candida.

Candida ID agar allows identification of Candida albicans and differentiation of other Candida species. In comparison with CHROMagar Candida, we evaluated the performance of this medium directly from 596 clinical specimens. In particular, detection of C. albicans after 24 h of incubation was easier on Candida ID (sensitivity, 96.8%) than on CHROMagar (sensitivity, 49.6%).

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Hemolytic activities of the Candida species in liquid medium.

OBJECTIVE The aim of this study was to evaluate the in vitro hemolytic activities of 107 Candida strains isolated from different clinical samples in liquid medium, and to examine the impact of glucose on this activity. MATERIALS AND METHODS A total of 107 Candida isolates representing seven species (C. albicans, n=28; C. glabrata, n=23; C. tropicalis, n=17; C. parapsilosis, n=16; C. kefyr, n=...

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ژورنال

عنوان ژورنال: Applied Microbiology

سال: 1966

ISSN: 0003-6919

DOI: 10.1128/am.14.4.525-528.1966