Modified TMV Particles as Beneficial Scaffolds to Present Sensor Enzymes

Modified TMV Particles as Beneficial Scaffolds to Present Sensor Enzymes

Tobacco mosaic virus (TMV) is a robust nanotubular nucleoprotein scaffold increasingly employed for the high density presentation of functional molecules such as peptides, fluorescent dyes, and antibodies. We report on its use as advantageous carrier for sensor enzymes. A TMV mutant with a cysteine residue exposed on every coat protein (CP) subunit (TMVCys) enabled the coupling of bifunctional ...

Supplementary Material Modified TMV particles as beneficial scaffolds to present sensor enzymes

Functional self-assembling bolaamphiphilic polydiacetylenes as colorimetric sensor scaffolds.

Conjugated polymers capable of responding to external stimuli by changes in optical, electrical, or electrochemical properties can be used for the construction of direct sensing devices. Polydiacetylene-based systems are attractive for sensing applications due to their colorimetric response to changes in the local environment. Here we present the design, preparation, and characterization of sel...

Penicillin Detection by Tobacco Mosaic Virus-Assisted Colorimetric Biosensors

The presentation of enzymes on viral scaffolds has beneficial effects such as an increased enzyme loading and a prolonged reusability in comparison to conventional immobilization platforms. Here, we used modified tobacco mosaic virus (TMV) nanorods as enzyme carriers in penicillin G detection for the first time. Penicillinase enzymes were conjugated with streptavidin and coupled to TMV rods by ...

Alumina nanoparticles modified carbon paste electrode as a new voltammetric sensor for determination of dopamine

The present study examines a new dopamine sensor based on Alumina nanoparticles modified carbon paste electrode (Al2O3NPsCPE). Moreover, the present study focuses on the electrochemical act of the Al2O3NPsCPE for the detection of dopamine by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). There is also a focus on the specification of the prepared modified electrode by electroc...