Molecular Modeling of Cloned Bacillus subtilis Keratinase and Its Insinuation in Psoriasis Treatment Using Docking Studies

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Characterization of the keratinolytic activity of indigenous Bacillus subtilis keratinase

Keratins are water insoluble proteins of our environment. Being extremely resistant to degradation by proteolytic enzymes, keratins are digested mainly by alkali and keratinase enzymes. The aim of present study was to characterize the indigenous keratinase for potentials of keratin-degrading activities. We report the purification and characterization of keratinase from Bacillus subtilis isolate...

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Novel keratinase from Bacillus subtilis S14 exhibiting remarkable dehairing capabilities.

We report the isolation of a keratinolytic-producing Bacillus subtilis strain and the characterization of the exceptional dehairing properties of its subtilisin-like keratinase. This enzyme can be an alternative to sodium sulfide, the major pollutant from tanneries, and may completely replace it. Its unique nonactivity upon collagen enhances its industrial potential.

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optimization of keratinase production for feather degradation by bacillus subtilis

results the pcr approved the bacillus genus of the isolates. the strain of bacillus subtilis was identified using biochemical tests. 40 ºc and ph 11 are the optimum condition for maximum keratinase enzyme activity. objectives the aim of this study was the isolation of feather degrading bacillus spp.from a poultry waste and the optimization of conditions for the highest enzyme activity and feath...

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MOLECULAR CLONING AND EVALUATION OF WILD PROMOTER IN EXPRESSION OF BACILLUS SPHAERICUS PHENYLALANINE DEHYDROGENASE GENE IN BACILLUS SUBTILIS CELLS

To evaluate the role of wild promoter of L-phenylalanine dehydrogenase (PheDH) gene, referred to as pdh, from Bacillus sphaericus in expression, cloning of pdh gene in Bacillus subtilis was performed. The whole pdh gene was cloned in pHY300PLK shuttle vector and amplified, construct (pHYDH) then transformed in B. subtilis ISW1214 and E. coli JM109. The pdh endogenous promoter presented no effec...

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ژورنال

عنوان ژورنال: Indian Journal of Microbiology

سال: 2017

ISSN: 0046-8991,0973-7715

DOI: 10.1007/s12088-017-0677-x