Monitoring human cytomegalovirus viral load in peripheral blood leukocytes of renal transplant recipients by a simple limiting dilution-PCR assay
نویسندگان
چکیده
منابع مشابه
Monitoring human cytomegalovirus viral load in peripheral blood leukocytes of renal transplant recipients by a simple limiting dilution-PCR assay.
To assess the clinical relevance of a semi-quantitative measurement of human cytomegalovirus (HCMV) DNA in renal transplant recipients within the typical clinical context of a developing country where virtually 100% of both receptors and donors are seropositive for this virus, we have undertaken HCMV DNA quantification using a simple, semi-quantitative, limiting dilution polymerase chain reacti...
متن کاملCytomegalovirus infection in renal transplant recipients diagnosed by nested-PCR.
A prospective study of cytomegalovirus (CMV) infection was carried out on 34 renal transplant recipients managed at a General Hospital in Ribeirão Preto, SP, Brazil. Serologic tests showed that all patients were infected with CMV before renal transplantation. Two nested-PCR techniques with primers that recognize sequences of the glycoprotein B (gB) and H (gH) genes were used for CMV detection i...
متن کاملMonitoring of cytomegalovirus infection in solid-organ transplant recipients by an ultrasensitive plasma PCR assay.
Early and accurate monitoring of cytomegalovirus (CMV) infection in solid-organ transplant recipients is of major importance. We have assessed the potential benefit of an ultrasensitive plasma-based PCR assay for renal transplant recipients. The pp65 CMV antigen (pp65 Ag) assay using leukocytes was employed as a routine test for the monitoring of CMV in 23 transplant recipients. We compared the...
متن کاملQuantitative Analysis of CMV-DNA Load in Renal Transplant Recipients Using Real-Time PCR
No abstract
متن کاملpre-symptomatic human cytomegalovirus disease diagnosis in renal transplant recipients by the virus dna pcr
human cytomegalovirus (hcmv, cmv) is a major infectious complication of renal transplantation. the objective of this survey was to optimize and establish a polymerase chain reaction (pcr) technique for rapid and early detection of cmv disease in renal transplant recipients. in a cross sectional study, a total of eighty-one edta-blood samples were collected as simple nonrandomized (sequential) w...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Brazilian Journal of Medical and Biological Research
سال: 1999
ISSN: 0100-879X
DOI: 10.1590/s0100-879x1999001200010