Monoclonal antibody fragment removal mediated by mixed mode resins

DNA binding mode of the Fab fragment of a monoclonal antibody specific for cyclobutane pyrimidine dimer.

Monoclonal antibodies specific for the cyclobutane pyrimidine dimer (CPD) are widely used for detection and quantification of DNA photolesions. However, the mechanisms of antigen binding by anti-CPD antibodies are little understood. Here we report NMR analyses of antigen recognition by TDM-2, which is a mouse monoclonal antibody specific for the cis - syn -cyclobutane thymine dimer (T[ c, s ]T)...

Reversing EGFR Mediated Immunoescape by Targeted Monoclonal Antibody Therapy

Uncontrolled growth is a signature of carcinogenesis, in part mediated by overexpression or overstimulation of growth factor receptors. The epidermal growth factor receptor (EGFR) mediates activation of multiple oncogenic signaling pathways and escape from recognition by the host immune system. We discuss how EGFR signaling downregulates tumor antigen presentation, upregulates suppressive check...

Identification of the membrane receptor for the complement fragment C3d by means of a monoclonal antibody

The B2 antigen characterized by means of a monoclonal antibody (14) is a 140,000 Mr protein expressed only in certain stages of the differentiation of lymphocytes of the B lineage. Here we examine the relationship between B2 and the membrane complement receptor type 2 (CR2) for the complement fragment C3d (11, 12), which is also associated only with B cells. Both phenotypic markers are distribu...

Removal of Copper from Aqueous Solution by Ion Exchange Resins

Customer Application Brief Removal Of Host Cell Proteins By Zeta PlusTM Filters During Monoclonal Antibody Purifi

Removal Of Host Cell Proteins By Zeta PlusTM Filters During Monoclonal Antibody Purifi cation Introduction Protein A chromatography is a widely used and highly successful method of purifying recombinant monoclonal antibodies. However, since Protein A has high affi nity for antibodies, harsh conditions such as low pH are often required to elute monoclonal antibodies from Protein A affi nity colu...