Monofunctional pyrenes at carbon nanotube electrodes for direct electron transfer H2O2 reduction with HRP and HRP-bacterial nanocellulose

The non-covalent modification of carbon nanotube electrodes with pyrene derivatives is a versatile approach to enhance the electrical wiring enzymes for biosensors and biofuel cells. We report here comparative study five adsorbed at multi-walled shed light on their ability promote direct electron transfer horseradish peroxidase (HRP) H 2 O reduction. In all cases, pyrene-modified enhanced catalytic reduction compared unmodified electrodes. N-hydroxysuccinimide (NHS) ester derivative provided access highest current 1.4 mA cm −2 6 mmol L −1 , high onset potential 0.61 V vs. Ag/AgCl, insensitivity parasitic oxidation, large linear dynamic range that benefits from HRP “suicide inactivation” 4–6 . Pyrene-aliphatic carboxylic acid groups offer better sensor sensitivity higher currents ≤ 1 concentrations. butyric NHS gave analytical sensitivities 5.63 A M 2.96 respectively, over wide (0.25–4 ) existing carbon-based biosensor bacterial nanocellulose pyrene-NHS bioelectrode was subsequently elaborated via “one-pot” “layer-by-layer” strategies. optimised exhibited slightly weaker voltage output, further currents, major enhancement in 1-week stability 67% activity remaining 39% equivalent electrode without nanocellulose, thus offering excellent prospects biosensing cell applications. • Simple bioelectrodes by adsorption pyrenes enhances catalysis. Hydrogen peroxide boost effect observed substrate concentration. 5 are biocathode performance. More eco-friendly integrating storage stability.