Monosaccharide transport into lactating-rat mammary acini
نویسندگان
چکیده
منابع مشابه
Lipogenesis by acini from mammary gland of lactating rats.
Intact acini were prepared from the mammary gland of lactating rats by treatment with collagenase. The acini were obtained in high yield. The preparation offers considerable advantage over the use of tissue slices. The acini were incubated with glucose, lactate, pyruvate, and acetate labeled with 14C and tritium, as single substrates or in combination with glucose. Synthesis of fatty acids from...
متن کاملCa2+ transport and Ca2+-dependent ATP hydrolysis by Golgi vesicles from lactating rat mammary glands.
Ca2+ transport across mammary-gland Golgi membranes was measured after centrifugation of the membrane vesicles through silicone oil. In the presence of 2.3 microM free Ca2+ the vesicles accumulated 5.8 nmol of Ca2+/mg of protein without added ATP, and this uptake was complete within 0.5 min. In the presence of 1 mM-ATP, Ca2+ was accumulated at a linear rate for 10 min after the precipitation of...
متن کاملRegulation of MonosacchaRide tRanspoRt
An additional monosaccharide transport pathway stems from the interesting findings that GLUT2, the classic facilitative glucose transporter initially believed to be sequestered to the basolateral membrane, as well as a novel facilitative glucose-specific transporter GLUT7 (SLC2A7), can both be inserted into the brush border in response to high glucose loads in the lumen of the small intestine.1...
متن کاملPeptide Transport and Metabolism by the Lactating Mammary Gland.
There has been considerable interest in the metabolism of small peptides by the lactating mammary gland since the discovery that plasma-derived peptides may be an important source of amino nitrogen for milk protein synthesis (1). The uptake of certain essential amino acids is less than their output in milk protein, and evidence suggests that the shortfall is made up from peptide-derived amino a...
متن کاملIncorporation into phospholipid vesicles of pore-like properties from Golgi membranes of lactating-rat mammary gland.
The ability of rat mammary-gland Golgi membranes to produce monosaccharide-specific pores in phospholipid vesicles was investigated. The apparent ability of Triton X-100 extracts of Golgi membranes to form such pores was re-evaluated, since we have now found that an apparent pore is produced by the detergent alone. We therefore incorporated intact Golgi membranes (1 mg of protein) into egg-yolk...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1982
ISSN: 0306-3283
DOI: 10.1042/bj2040493