Morphogenic Protein RodZ Interacts with Sporulation Specific SpoIIE in Bacillus subtilis

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Morphogenic Protein RodZ Interacts with Sporulation Specific SpoIIE in Bacillus subtilis

The first landmark in sporulation of Bacillus subtilis is the formation of an asymmetric septum followed by selective activation of the transcription factor σF in the resulting smaller cell. How the morphological transformations that occur during sporulation are coupled to cell-specific activation of transcription is largely unknown. The membrane protein SpoIIE is a constituent of the asymmetri...

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Interaction of the Morphogenic Protein RodZ with the Bacillus subtilis Min System

Vegetative cell division in Bacillus subtilis takes place precisely at the middle of the cell to ensure that two viable daughter cells are formed. The first event in cell division is the positioning of the FtsZ Z-ring at the correct site. This is controlled by the coordinated action of both negative and positive regulators. The existence of positive regulators has been inferred, but none have p...

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Evaluation of the kinetic properties of the sporulation protein SpoIIE of Bacillus subtilis by inclusion in a model membrane.

Starvation induces Bacillus subtilis to initiate a developmental process (sporulation) that includes asymmetric cell division to form the prespore and the mother cell. The integral membrane protein SpoIIE is essential for the prespore-specific activation of the transcription factor sigmaF, and it also has a morphogenic activity required for asymmetric division. An increase in the local concentr...

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Purification, kinetic properties, and intracellular concentration of SpoIIE, an integral membrane protein that regulates sporulation in Bacillus subtilis.

SpoIIE is a bifunctional protein which controls sigmaF activation and formation of the asymmetric septum in sporulating Bacillus subtilis. The spoIIE gene of B. subtilis has now been overexpressed in Escherichia coli, and SpoIIE has been purified by anion-exchange chromatography and affinity chromatography. Kinetic studies showed that the rate of dephosphorylation of SpoIIAA-P by purified SpoII...

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Methionine sulfoxide and specific inhibition of sporulation in Bacillus subtilis.

Methionine sulfoxide has been shown to be a specific antagonist of glutamic acid in the conversion of glutamic acid to glutamine by Waelsch, Owades, Miller, and Borek (J. Biol. Chem., 166, 273, 1946) in growth inhibition studies with Lactobacillus arabinosus. Elliot and Gale (Nature, 161, 129, 1948) using cell-free extracts of Staphylococcus aureus have demonstrated that methionine sulfoxide in...

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ژورنال

عنوان ژورنال: PLOS ONE

سال: 2016

ISSN: 1932-6203

DOI: 10.1371/journal.pone.0159076