Morphologic probes of polypeptide hormone receptor interactions.
نویسندگان
چکیده
منابع مشابه
Thyroxine-thyroid hormone receptor interactions.
Thyroid hormone (TH) actions are mediated by nuclear receptors (TRs alpha and beta) that bind triiodothyronine (T(3), 3,5,3'-triiodo-l-thyronine) with high affinity, and its precursor thyroxine (T(4), 3,5,3',5'-tetraiodo-l-thyronine) with lower affinity. T(4) contains a bulky 5' iodine group absent from T(3). Because T(3) is buried in the core of the ligand binding domain (LBD), we have predict...
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The advent of ultramicroscale purification and analysis technology, along with gene manipulation techniques, has greatly accelerated progress in molecular cell biology. (We used to call this "Biochemistry.") Nowhere is the rate of progress more impressively evident than in the field of hormone receptor study, where the enthusiastic research of numerous investigators is rapidly clarifying struct...
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The thyroid hormone receptor (TR) directly regulates the transcription of thyroid hormone-responsive genes in response to changing levels of thyroid hormone. Mechanistically TR utilizes a complex set of binding interactions, with hormone, response elements, and coregulatory proteins, to provide specific local control of patterns of transcriptional response that are partially responsible for ind...
متن کاملInvestigation of hormone-receptor interactions by means of fluorescence labeling.
Fluorescent-labeled hormones can be used to study hormone-receptor interactions by means of fluorescence polarization, visualization by fluorescence microscopy, or separation methods, e.g., dextran-coated charcoal. Subcellular fragments, single cells, and tissue preparations are amenable to study; in this work rat uterine cytosol was used unless otherwise noted. Estrone labeled with fluorescein...
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To define the sites in the extracellular domain of the human thyrotropin (TSH) receptor that are involved in TSH binding and signal transduction we constructed chimeric thyrotropin-luteinizing hormone/chorionic gonadotropin (TSH-LH/CG) receptors. The extracellular domain of the human TSH receptor was divided into five regions that were replaced, either singly or in various combinations, with ho...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1980
ISSN: 0022-1554,1551-5044
DOI: 10.1177/28.8.6255030