Multiple forms of cytochrome P-450 in diabetic rats
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چکیده
منابع مشابه
Multiple Forms of Cytochrome P-450 in Phenobarbital- and 3-Methylcholanthrene-treated Rats
Multiple forms of liver microsomal cytochrome P-450 isolated from immature male rats pretreated with phenobarbital or 3-methylcholanthrene are described. A fraction. of low specific content (Fraction A, 1.7 to 4.0 nmol of cytochrome P-450 per mg of protein) and a fraction substantially purified (Fraction B, 9.0 to 11.0 mnol of cytochrome P-450 per mg of protein) are obtained by DEAE-cellulose c...
متن کاملSeparation and purification of multiple forms of microsomal cytochrome P-450. Activities of different forms of cytochrome P-450 towards several compounds of environmental interest.
A new procedure has been devised for the separation and purification of the cytochromes P-450 from the hepatic microsomes of male rats, based upon the initial procedures of Imai and Sato (Imai, Y., and Sato, K. (1974) Riochem. Biophys. Res. Commun. 60, 8-14). Isolated fractions range in nominal specific content (nanomoles of cytochrome P-450 per mg of protein) from 6.8 to 15.4 for phenobarbital...
متن کاملMultiple forms of cytochrome P-450 in phenobarbital- and 3-methylcholanthrene-treated rats. Separation and spectral properties.
Multiple forms of liver microsomal cytochrome P-450 isolated from immature male rats pretreated with phenobarbital or 3-methylcholanthrene are described. Afraction of low specific content (Fraction A. 1.7 TO 4.0 nmol of cytochrome P-450 per mg of protein) and a fraction substantially purified (Fraction B, 9.0 TO 11.0 NMOL of cytochrome P-450 per mg of protein) are obtained by DEAE-cellulose chr...
متن کاملCatalysis of divergent pathways of 2-acetylaminofluorene metabolism by multiple forms of cytochrome P-450.
Four highly purified forms of rabbit hepatic, microsomal cytochrome P-450 catalyze the N- and ring-hydroxylation of 2-acetylaminofluorene (AAF) at different rates. Form 4, the major form of the cytochrome induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in adult rabbit liver, catalyzed the N-hydroxylation of AAF more rapidly than did the other three forms. N-Hydroxy-2-acetylaminofluorene accounte...
متن کاملPurified liver microsomal cytochrome P-450. Separation and characterization of multiple forms.
During the purification of rabbit liver microsomal cytochrome P-450 (P-450LM), evidence was obtained for the occurrence of at least four distinct forms. These were distinguished by polyacrylamide gel electrophoresis after treatment with sodium dodecyl sulfate in the presence or absence of mercaptoethanol and were shown to have characteristic spectra as the reduced carbon monoxide complexes. The...
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ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1986
ISSN: 0021-5198
DOI: 10.1016/s0021-5198(19)58949-1