Multiple forms of rat kidney mutarotase.
نویسندگان
چکیده
منابع مشابه
Multiple Forms of Rat Liver Monoamine Oxidase
1. Treatment of a partly purified preparation of rat liver monoamine oxidase with the chaotropic agent sodium perchlorate caused the enzyme to migrate as a single band of activity on polyacrylamide-gel electrophoresis, whereas the untreated enzyme separated into a number of bands. 2. Treatment with the chaotropic agent caused no loss ofenzyme activity towards benzylamine, dopamine or tyramine. ...
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1. Ten rat tissues and organs have been assayed for ,-galactosidase with phenyl ,B-D-galactoside, p-nitrophenyl B-D-galactoside, p-aminophenyl ,B-D-galactoside and 4-methylumbelliferyl fl-D-galactoside as substrates. 2. The relative activities of these tissues are independent ofthe mode ofassay, andmaximum rates ofhydrolysis are not greatly affected by the nature ofthe substrate. 3. Inhibition ...
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Galactose mutarotase catalyzes the conversion of beta-d-galactose to alpha-d-galactose during normal galactose metabolism. The enzyme has been isolated from bacteria, plants, and animals and is present in the cytoplasm of most cells. Here we report the x-ray crystallographic analysis of human galactose mutarotase both in the apoform and complexed with its substrate, beta-d-galactose. The polype...
متن کاملThe catalytic mechanism of galactose mutarotase.
Galactose mutarotase catalyzes the first step in normal galactose metabolism by catalyzing the conversion of beta-D-galactose to alpha-D-galactose. The structure of the enzyme from Lactococcus lactis was recently solved in this laboratory and shown to be topologically similar to domain 5 of beta-galactosidase. From this initial X-ray analysis, four amino acid residues were demonstrated to be in...
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ژورنال
عنوان ژورنال: Chemical and Pharmaceutical Bulletin
سال: 1976
ISSN: 0009-2363,1347-5223
DOI: 10.1248/cpb.24.2893