Multiple Transcripts for Rat Nucleoside Diphosphate Kinase α Isoform Are Structurally Categorized into Two Groups That Exhibit Cell-specific Expression and Distinct Translation Potential
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Rat pancreatic nucleoside diphosphate kinase, a novel regulator of cholecystokinin receptor affinity. Cloning and expression.
We previously demonstrated that the existence of two affinity states of the pancreatic cholecystokinin (CCK) receptor depends on the presence of ATP.' Subsequently, we established that the effect of ATP to induce two CCK binding affinity states was biochemically mediated by the enzyme nucleoside diphosphate kinase (NDPK) (manuscript in preparation). Nucleoside diphosphate kinase catalyzes the t...
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A low molecular mas (18 kD) phosphoprotein (pp18) was characterized and purified from cultured sugarcane (Saccharum officinarum 1.) cell line H50-7209. Autophosphorylation assays were used to detect pp18 after separation by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PACE). Only pp18 was detected by a brief in situ phosphorylation method, whereas additional putative protein ki...
متن کاملNucleoside diphosphate kinase from Escherichia coli.
Nucleoside diphosphate (NDP) kinase from Escherichia coli was purified to homogeneity and was crystallized. Gel filtration analysis of the purified enzyme indicated that it forms a tetramer. The enzyme was phosphorylated with [gamma-32P]ATP, and the pH stability profile of the phosphoenzyme indicated that two different amino acid residues were phosphorylated. Both a histidine residue and serine...
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Nucleoside diphosphate (NDP) kinase has been postulated to generate GTP from the GDP bound to tubulin. The purified chick brain enzyme was studied with respect to its kinetic parameters, and the protein-protein interactions between the NDP kinase and tubulin were examined. No specific interaction is observed between the enzyme and assembled microtubules, tubulin dimers, or tubulin-microtubule-a...
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Nucleoside diphosphate (NDP) kinase is a ubiquitous nonspecific enzyme that evidently is designed to catalyze in vivo ATP-dependent synthesis of ribo- and deoxyribonucleoside triphosphates from the corresponding diphosphates. Because Escherichia coli contains only one copy of ndk, the structural gene for this enzyme, we were surprised to find that ndk disruption yields bacteria that are still v...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1997
ISSN: 0021-9258
DOI: 10.1074/jbc.272.6.3289