Multiplex PCR: advantages, development, and applications.
Institute for Molecular Genetics, Baylor College of Medicine, Houston, Texas 77030 Ever since it was shown that PCR could simultaneously amplify multiple loci in the human dystrophin gene, (1) multiplex PCR has been firmly established as a general technique. A short list of multiplex PCR applications now includes pathogen identification, gender screening, linkage analysis, forensic studies, tem...متن کامل
Extensive diagnostic and scientific investigations are often restricted by limited availability of material. Therefore, methods like multiplex PCR strategies are needed to conserve as much sample as possible. Unfortunately, the establishment of such procedures poses several difficulties. Here we describe the advantages of a new enzyme, AmpliTaq Gold DNA Polymerase, in multiplex and time-release...متن کامل
Multiplex PCR method is a powerful technology as a potent tool to amplify specific DNA fragments from genomic DNA using Taq DNA polymerase and primer sets. Since multiple reactions occur in single tube, multiplex PCR has the potential to produce considerable savings of time and effort. Its applications are widely ranged from in vitro cloning of specific regions of DNA to diagnosis of disease [1...متن کامل
A simple molecular tool was developed and tested to identify seven mealybug species found in North American vineyards: Pseudococcus maritimus Ehrhorn, Pseudococcus viburni (Signoret), Pseudococcus longispinus (Targioni-Tozzeti), Pseudococcus calceolariae (Maskell), Planococcus ficus (Signoret), Planococcus citri (Risso), and Ferrisia gilli Gullan. The developed multiplex PCR is based on the mit...متن کامل