Multiplex PCR Assay for Simultaneous Detection of Korean Quarantine Phytoplasmas
Real-time PCR for simultaneous and quantitative detection of quarantine phytoplasmas from apple proliferation (16 SrX) group.
A real time PCR assay conjugated with the fluorescent SYBR Green I dye has been developed for rapid, sensitive and quantitative detection of 'Ca. Phytoplasma pyri', 'Ca. P. prunorum' and 'Ca. P. mali', quarantine members of apple proliferation (16 SrX) group. The selected primers amplify specifically a target of 217-bp fragment from the 16 Sr gene region of the 16 SrX group and not from any oth...متن کامل
The importation of sugarcane varieties from other countries is necessary for the breeding of new varieties suited to the diverse environmental conditions in South Africa. To prevent the introduction and spread of new diseases, it is crucial that any imported pathogens are detected before the cane is released from quarantine. Although not present in South Africa, Grassy Shoot (GS) and Sugarcane ...متن کامل
Development of a Multiplex TaqMan Real-Time PCR Assay for Sensitive and Rapid Detection of Phytoplasmas Infecting Rubus Species
Rubus stunt, a disease associated with phytoplasma infections in wild and cultivated Rubus species, is a major challenge in the production of raspberries, blackberries, and loganberries. Phytoplasmas are cell wall-less bacteria inhabiting the phloem and are transferred by phloem feeding insects. As the time between infection of a plant and the development of disease symptoms can take up to 1 ye...متن کامل
Development of a bead-based multiplex PCR assay for the simultaneous detection of multiple Mycoplasma species.
We describe the development and analytical validation of a 7-plex polymerase chain reaction assay coupled to a bead-based liquid suspension array for detection of multiple ruminant Mycoplasma spp. The assay employs a combination of newly designed and previously validated primer-probe sets that target genetic loci specific for Mycoplasma bovis, Mycoplasma mycoides cluster, Mycoplasma mycoides su...متن کامل
Multiplex Real-Time qPCR Assay for Simultaneous and Sensitive Detection of Phytoplasmas in Sesame Plants and Insect Vectors
Phyllody, a destructive and economically important disease worldwide caused by phytoplasma infections, is characterized by the abnormal development of floral structures into stunted leafy parts and contributes to serious losses in crop plants, including sesame (Sesamum indicum L.). Accurate identification, differentiation, and quantification of phyllody-causing phytoplasmas are essential for ef...متن کامل
عنوان ژورنال: The Plant Pathology Journal