Multiplex Real-Time PCR for Rapid Staphylococcal Cassette Chromosome mec Typing
نویسندگان
چکیده
منابع مشابه
Novel PCR-restriction fragment length polymorphism analysis for rapid typing of staphylococcal cassette chromosome mec elements.
We developed a novel PCR-restriction fragment length polymorphism test for the ccrB gene by using HinfI and BsmI for rapid typing of staphylococcal cassette chromosome mec (SCCmec). When tested with reference strains and methicillin-resistant Staphylococcus aureus isolates, the method proved to be valid and useful for rapid identification of four SCCmec types, especially type IV.
متن کاملEnhanced multiplex PCR assay for typing of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus.
We previously published a simple multiplex PCR assay for quick-screening of Staphylococcal Cassette Chromosome mec (SCCmec) types I-V, which has been extensively used worldwide. An updated assay is described here and the changes serve to make this rapid assay more accurate and reliable in facilitating identification of most common and major SCCmec types.
متن کاملGenetic diagnosis of community-acquired MRSA: a multiplex real-time PCR method for Staphylococcal cassette chromosome mec typing and detecting toxin genes.
Methicillin-resistant Staphylococcus aureus (MRSA) causes a wide range of infections in health care settings and community environments. In particular, community-acquired MRSA (CA-MRSA) is important for clinicians because many fatal cases in healthy populations have been reported. Staphylococcal cassette chromosome mec (SCCmec) is a mobile genetic element and carries the central determinant for...
متن کاملStaphylococcal Cassette Chromosome mec in MRSA, Taiwan
To determine the predominant staphylococcal cassette chromosome (SCC) mec element in methicillin-resistant Staphylococcus aureus, we typed 190 isolates from a hospital in Taiwan. We found a shift from type IV to type III SCCmec element during 1992-2003, perhaps caused by selective pressure from indiscriminate use of antimicrobial drugs.
متن کاملSystematic derivation of marker sets for staphylococcal cassette chromosome mec typing.
The aim of this study was to identify optimized sets of genotyping targets for the staphylococcal cassette chromosome mec (SCCmec). We analyzed the gene contents of 46 SCCmec variants in order to identify minimal subsets of targets that provide useful resolution. This was achieved by firstly identifying and characterizing each available SCCmec element based on the presence or absence of 34 bina...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2009
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.00766-09