Multivariable optimization process of heterotrophic growth of Chlorella vulgaris

Microalgae have received increasing attention as one of the most promising feedstocks in development new healthier food products and different strategies been attempted to improve their growth. However, high production costs low productivities, commonly associated with photoautotrophic growths, are still a big challenge. In this study, two-step optimization strategy was carried out order maximize biomass Chlorella vulgaris strain used at industrial scale under heterotrophic conditions. From total 24 independent variables, which were studied simultaneously, 10 presented positive effect over Xmax, while remaining shown be negative. The amount (NH4)2SO4 (6.3 g L−1), MgSO4·7H2O (0.7 C6H12O6 (50% w/v) culture medium has revealed only factors significant impact on concentration, optimum values 25.5, 64.6, 75 ml.L−1, respectively. optimized resulted an improvement Xmax by 99.6% when compared growth applied scale, proving success strategy. Additionally, carbohydrates enhanced 48.0%.