Muscle Transcriptome Analysis Reveals Molecular Mechanisms of Superior Growth Performance in Kuruma Shrimp, Marsupenaeus japonicus

During the cultivation of Marsupenaeus japonicus, there are often obvious differences in growth within same family under food, water quality, and environment, which greatly affects efficiency. To explore molecular mechanism this difference, study used RNA-seq technology to compare transcriptomes M. japonicus individuals with significant from family. A total 1375 differentially expressed genes were identified, 1109 upregulated 266 downregulated. Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) analyses performed on genes, results showed that growth-related processes, including chitin metabolism, chitin-binding amino sugar antioxidant response oxidative stress, peroxidase activity, between large-size small-size groups. The expression levels some such as cuticular protein, low-density lipoprotein receptor, ecdysteroid kinase, myosin heavy chain, apoptosis inhibitor, verified by quantitative PCR experiments. One cuticle gene was annotated, phylogenetic analysis sequence clustered penaeid genes. This provides valuable data a scientific basis for understanding at molecular-genetic level.