Mutational analysis using oligonucleotide microarrays
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چکیده
منابع مشابه
Mutational analysis using oligonucleotide microarrays.
The development of inexpensive high throughput methods to identify individual DNA sequence differences is important to the future growth of medical genetics. This has become increasingly apparent as epidemiologists, pathologists, and clinical geneticists focus more attention on the molecular basis of complex multifactorial diseases. Such undertakings will rely upon genetic maps based upon newly...
متن کاملReview article Mutational analysis using oligonucleotide microarrays
The development of inexpensive high throughput methods to identify individual DNA sequence diVerences is important to the future growth of medical genetics. This has become increasingly apparent as epidemiologists, pathologists, and clinical geneticists focus more attention on the molecular basis of complex multifactorial diseases. Such undertakings will rely upon genetic maps based upon newly ...
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Although oligonucleotide probes complementary to single nucleotide substitutions are commonly used in microarray-based screens for genetic variation, little is known about the hybridization properties of probes complementary to small insertions and deletions. It is necessary to define the hybridization properties of these latter probes in order to improve the specificity and sensitivity of olig...
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Fly stocks Fly culture and crosses were grown on standard fly medium at 25 8C. Oregon R were used as wild-type standard. The BG00650 line was obtained from the Bloomington stock centre. Dredd , Relish, Dif 1 and imd were described elsewhere. The P-element inserted in BG00650 was mobilized by following standard crosses. Mobilization events were screened by PCR using different primer combinations...
متن کاملHigh-resolution analysis of DNA copy number using oligonucleotide microarrays.
Genomic copy number alterations are a feature of many human diseases including cancer. We have evaluated the effectiveness of an oligonucleotide array, originally designed to detect single-nucleotide polymorphisms, to assess DNA copy number. We first showed that fluorescent signal from the oligonucleotide array varies in proportion to both decreases and increases in copy number. Subsequently we...
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ژورنال
عنوان ژورنال: Journal of Medical Genetics
سال: 1999
ISSN: 0022-2593,1468-6244
DOI: 10.1136/jmg.36.10.730