Mutations in the substrate entrance region of -glucosidase from Trichoderma reesei improve enzyme activity and thermostability
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چکیده
منابع مشابه
Increased Production and Activity of Cellulase Enzyme of Trichoderma reesei by Using Gibberellin Hormone
Cellulolytic complex are enzymes capable of hydrolyzing cellulose. Due to rapid growth in population and industrialization, most countries are required to produce more fuel. Production of bioethanol from lignocellulosic biomass is very challenging due to environmental pollution by fossil fuels. Cellulases play a significant role in biotechnological processes. The cost of production of cellulase...
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چکیده ندارد.
15 صفحه اولEnzymatic properties and intracellular localization of the novel Trichoderma reesei beta-glucosidase BGLII (cel1A).
This paper describes the characterization of an intracellular beta-glucosidase enzyme BGLII (Cel1a) and its gene (bgl2) from the cellulolytic fungus Trichoderma reesei (Hypocrea jecorina). The expression pattern of bgl2 is similar to that of other cellulase genes known from this fungus, and the gene would appear to be under the control of carbon catabolite repression mediated by the cre1 gene. ...
متن کاملImprovements in Glucose Sensitivity and Stability of Trichoderma reesei β-Glucosidase Using Site-Directed Mutagenesis
Glucose sensitivity and pH and thermal stabilities of Trichoderma reesei Cel1A (Bgl II) were improved by site-directed mutagenesis of only two amino acid residues (L167W or P172L) at the entrance of the active site. The Cel1A mutant showed high glucose tolerance (50% of inhibitory concentration = 650 mM), glucose stimulation (2.0 fold at 50 mM glucose), and enhanced specific activity (2.4-fold)...
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ژورنال
عنوان ژورنال: Protein Engineering Design and Selection
سال: 2012
ISSN: 1741-0126,1741-0134
DOI: 10.1093/protein/gzs073