Na/K pump current in aggregates of cultured chick cardiac myocytes.
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چکیده
منابع مشابه
Na/K pump current in aggregates of cultured chick cardiac myocytes
Spontaneously beating aggregates of cultured embryonic chick cardiac myocytes, maintained at 37 degrees C, were voltage clamped using a single microelectrode switching clamp to measure the current generated by the Na/K pump (Ip). In resting, steady-state preparations an ouabain-sensitive current of 0.46 +/- 0.03 microA/cm2 (n = 22) was identified. This current was not affected by 1 mM Ba, which...
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The measured apparent affinity (K0.5) of the Na/K pump for ouabain has been reported to vary over a wide range. In a previous report we found that changing Na~ could alter apparent affinity by at least an order of magnitude and that the model presented predicted this variability. To increase our understanding of this variability, isolated cells or twoto three-cell clusters of cardiac myocytes f...
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متن کاملApparent affinity of the Na/K pump for ouabain in cultured chick cardiac myocytes. Effects of Nai and Ko
The measured apparent affinity (K0.5) of the Na/K pump for ouabain has been reported to vary over a wide range. In a previous report we found that changing Nai could alter apparent affinity by at least an order of magnitude and that the model presented predicted this variability. To increase our understanding of this variability, isolated cells or two- to three-cell clusters of cardiac myocytes...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1990
ISSN: 0022-1295,1540-7748
DOI: 10.1085/jgp.95.1.61