Native Architecture of the Centriole Proximal Region Reveals Features Underlying Its 9-Fold Radial Symmetry
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چکیده
منابع مشابه
Native Architecture of the Centriole Proximal Region Reveals Features Underlying Its 9-Fold Radial Symmetry
BACKGROUND Centrioles are cylindrical microtubule-based structures whose assembly is critical for the formation of cilia, flagella, and centrosomes. The centriole proximal region harbors a cartwheel that dictates the 9-fold symmetry of centrioles. Although the cartwheel architecture has been recently analyzed, how it connects to the peripheral microtubules is not understood. More generally, a h...
متن کاملBld10p Constitutes the Cartwheel-Spoke Tip and Stabilizes the 9-Fold Symmetry of the Centriole
Centrioles/basal bodies have a characteristic cylindrical structure consisting of nine triplet microtubules arranged in a rotational symmetry. How this elaborate structure is formed is a major unanswered question in cell biology [1, 2]. We previously identified a 170 kDa coiled-coil protein essential for the centriole formation in Chlamydomonas. This protein, Bld10p, is the first protein shown ...
متن کاملSAS-6 is a Cartwheel Protein that Establishes the 9-Fold Symmetry of the Centriole
Centrioles consist of nine-triplet microtubules arranged in rotational symmetry. This structure is highly conserved among various eukaryotic organisms and serves as the base for the ciliary axoneme. Recently, several proteins such as SAS-6 have been identified as essential to the early process of centriole assembly, but the mechanism that produces the 9-fold symmetry is poorly understood. In C....
متن کاملSUPPORTING MATERIAL STED Microscopy with Optimized Labeling Density Reveals 9-fold Arrangement of a Centriole Protein
IMCD3 cells were grown in DME/F12 (Invitrogen) supplemented with 10% FBS at 37°C and 5% CO2. Mouse tracheal epithelial cell (MTEC) cultures were established as previously described (1, 2). Mice were sacrificed at 2–4 months of age, and trachea were excised, opened longitudinally to expose the lumen, and placed in 1.5 mg/ml pronase E in F12-Kaighn’s media (Invitrogen) at 4°C overnight. Tracheal ...
متن کاملSTED microscopy with optimized labeling density reveals 9-fold arrangement of a centriole protein.
Super-resolution fluorescence microscopy can achieve resolution beyond the optical diffraction limit, partially closing the gap between conventional optical imaging and electron microscopy for elucidation of subcellular architecture. The centriole, a key component of the cellular control and division machinery, is 250 nm in diameter, a spatial scale where super-resolution methods such as stimul...
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ژورنال
عنوان ژورنال: Current Biology
سال: 2013
ISSN: 0960-9822
DOI: 10.1016/j.cub.2013.06.061