Native flexibility of structurally homologous proteins: insights from anisotropic network model
نویسندگان
چکیده
منابع مشابه
Native flexibility of structurally homologous proteins: insights from anisotropic network model
BACKGROUND Single-molecule microscopic experiments can measure the mechanical response of proteins to pulling forces applied externally along different directions (inducing different residue pairs in the proteins by uniaxial tension). This response to external forces away from equilibrium should in principle, correlate with the flexibility or stiffness of proteins in their folded states. Here, ...
متن کاملModeling structurally variable regions in homologous proteins with rosetta.
A major limitation of current comparative modeling methods is the accuracy with which regions that are structurally divergent from homologues of known structure can be modeled. Because structural differences between homologous proteins are responsible for variations in protein function and specificity, the ability to model these differences has important functional consequences. Although existi...
متن کاملToward a molecular understanding of the anisotropic response of proteins to external forces: insights from elastic network models.
With recent advances in single-molecule manipulation techniques, it is now possible to measure the mechanical resistance of proteins to external pulling forces applied at specific positions. Remarkably, such recent studies demonstrated that the pulling/stretching forces required to initiate unfolding vary considerably depending on the location of the application of the forces, unraveling residu...
متن کاملFlexibility and Mobility in Mesophilic and Thermophilic homologous proteins from Molecular Dynamics and Foldunfold Method
To function properly protein molecules require both flexibility and rigidity, therefore fast and accurate prediction of protein rigidity/flexibility is one of the important problems in protein science. In this work we used two theoretical approaches to determine flexible regions in four homologous pairs of proteins from thermophilic and mesophilic organisms. Protein pairs chosen in this study w...
متن کاملIdentification of Local Conformational Similarity in Structurally Variable Regions of Homologous Proteins Using Protein Blocks
Structure comparison tools can be used to align related protein structures to identify structurally conserved and variable regions and to infer functional and evolutionary relationships. While the conserved regions often superimpose well, the variable regions appear non superimposable. Differences in homologous protein structures are thought to be due to evolutionary plasticity to accommodate d...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BMC Biophysics
سال: 2017
ISSN: 2046-1682
DOI: 10.1186/s13628-017-0034-9