Native Proteomics in Discovery Mode Using Size-Exclusion Chromatography–Capillary Zone Electrophoresis–Tandem Mass Spectrometry
نویسندگان
چکیده
منابع مشابه
Rapid characterization of biotherapeutic proteins by size-exclusion chromatography coupled to native mass spectrometry
High-molecular weight aggregates such as antibody dimers and other side products derived from incorrect light or heavy chain association typically represent critical product-related impurities for bispecific antibody formats. In this study, an approach employing ultra-pressure liquid chromatography size-exclusion separation combined with native electrospray ionization mass spectrometry for the ...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2018
ISSN: 0003-2700,1520-6882
DOI: 10.1021/acs.analchem.8b02725