Natural honey acts as a nonpermeating cryoprotectant for promoting bovine oocyte vitrification
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L-proline: a highly effective cryoprotectant for mouse oocyte vitrification
Recent studies have shown that L-proline is a natural osmoprotectant and an antioxidant to protect cells from injuries such as that caused by freezing and thawing in many species including plant, ram sperm and human endothelial cells. Nevertheless, this nontoxic cryoprotectant has not yet been applied to mammalian oocyte vitrification. In this study we evaluated the efficiency and safety of the...
متن کاملthe vitrification of metaphase ii mouse oocyte using ethylene glycol as cryoprotectant
introduction: the purpose of this study was to investigate the effect of vitrification procedure on the survival, fertilization, development and ultrastructure of metaphase (mii) mouse oocyte. materials and methods: female nmri mice 6-10 weeks old were superovulated using ip injection of 10 iu hmg and 10 iu hcg. ovulated oocyte were collected from the ampullary portion of the oviducts at 12-13 ...
متن کاملNanoliter droplet vitrification for oocyte cryopreservation.
AIM Oocyte cryopreservation remains largely experimental, with live birth rates of only 2-4% per thawed oocyte. In this study, we present a nanoliter droplet technology for oocyte vitrification. MATERIALS & METHODS An ejector-based droplet vitrification system was designed to continuously cryopreserve oocytes in nanoliter droplets. Oocyte survival rates, morphologies and parthenogenetic devel...
متن کاملThe effects of pre and post vitrification taxol treatment on bovine immature oocyte
The aim of the present study was to find the effects of pre and post vitrification taxol treatment on bovineimmature cumulus oocyte complexes (COCs). Bovine ovaries were collected from the local slaughterhousein a container with 30-37°C physiologic saline and supplemented with antibiotics. The grade A aspiratedCOCs were assigned to 6 experimental groups; The 1st group was control (n=100) in whi...
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ژورنال
عنوان ژورنال: PLOS ONE
سال: 2020
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0238573