Natural Transformation of Plasmid DNA in <i>Escherichia coli</i> in Sterilized Soil Column
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چکیده
منابع مشابه
Natural Transformation of Plasmid DNA in Escherichia coli in Sterilized Soil Column
Horizontal gene transfer allows rapid spread of antibiotic resistance genes1, which in bacteria inhabiting natural aquatic systems occurs by conjugation, transduction, and transformation2. Natural genetic transformation is characterized by the uptake of free DNA by a recipient bacterium, its chromosomal integration or extrachromosomal stabilization, and its expression, which leads to a new phen...
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Although Escherichia coli does not have a natural transformation process, strains of E. coli can incorporate extracellular plasmids into cytoplasm 'naturally' at low frequencies. A standard method was developed in which stationary phase cells were concentrated, mixed with plasmids, and then plated on agar plates with nutrients which allowed cells to grow. Transformed cells could then be selecte...
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The persistence and stability of free plasmid pUC8-ISP DNA introduced into 10-g samples of various soils and kept at 23 degrees C were monitored over a period of 60 days. The soils were sampled at a plant science farm and included a loamy sand soil (no. 1), a clay soil (no. 2), and a silty clay soil (no. 3). Four different methods allowed monitoring of (i) the production of acid-soluble radioac...
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A plasmid transformation system was developed for Bacillus polymyxa ATCC 12321 and derivatives of this strain. The method utilizes a penicillin-treated-cell technique to facilitate uptake of the plasmid DNA. Low-frequency transformation (10(-6) per recipient cell) of plasmids pC194, pBD64, and pBC16 was accomplished with this method. Selection for the transformants was accomplished on both hype...
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Azotobacter vinelandii cells can be transformed at high frequencies with the broad-host-range plasmids pRK2501, RSF1010, and pGSS15, using a modification of the procedure developed by Page and von Tigerstrom (J. Bacteriol. 139:1058-1061, 1979) for chromosomal DNA-mediated transformation. The frequency of transformation per microgram of plasmid DNA per viable cell with pRK2501 and pGSS15 was abo...
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ژورنال
عنوان ژورنال: Bangladesh Journal of Microbiology
سال: 1970
ISSN: 2408-8374,1011-9981
DOI: 10.3329/bjm.v25i1.4856