Near diffusion-controlled reaction of a Zn(Cys)4 zinc finger with hypochlorous acid

Near diffusion-controlled reaction of a Zn(Cys)4 zinc finger with hypochlorous acid

Univ. Grenoble Alpes, LCBM/PMB, F-38000 CNRS, LCBM/PMB, UMR 5249, F-38000 Gr CEA, BIG-CBM, PMB, F-38000 Grenoble, [email protected] Univ. Grenoble Alpes, INAC-SyMMES, F-38 CEA, INAC-SyMMES, F-38000 Grenoble, Fr † Electronic supplementary information concerning absorption, uorescence a oxidation of Zn$LHSP, identication experiments, re-assessment of rate con simulation of competitive HOCl...

On membrane diffusion with near-equilibrium reaction

An analysis is presented of the “facilitated” transport problem, involving steady diffusion through a finite membrane, accompanied by rapid and reversible, homogeneous chemical reaction. For the limit of infinitely fast reactions, the method of matched asymptotic expansions is used to analyze the asymptotic structure of the diffusion field, which is shown to consist of an “equilibrium core,” to...

Diffusion-Controlled Reaction Kinetics

Kinetic Studies on the Reaction between Dicyanocobinamide and Hypochlorous Acid

Hypochlorous acid (HOCl) is a potent oxidant generated by myeloperoxidase (MPO), which is an abundant enzyme used for defense against microbes. We examined the potential role of HOCl in corrin ring destruction and subsequent formation of cyanogen chloride (CNCl) from dicyanocobinamide ((CN)2-Cbi). Stopped-flow analysis revealed that the reaction consists of at least three observable steps, incl...

Reaction of hypochlorous acid with bovine nasal cartilage comparison to pig articular cartilage.

The action of sodium hypochlorite (NaOCl) on bovine nasal cartilage was studied by proton nuclear magnetic resonance (1H-NMR) spectroscopy in order to model degradation processes of cartilage caused by neutrophil-derived hypochlorous acid. Nasal cartilage was chosen as a mean of comparison because it differs from articular cartilage in its composition. It contains some more proteoglycans, i.e. ...